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未來(lái)已來(lái) | 全自動(dòng)柔性單細(xì)胞分離儀 Pala Q&A

[ 發(fā)布日期：2024-2-28 9:31:38 閱讀次數(shù)：747 ]

How Can Pala Help My Cell Sorting Workflows?

It’s time consuming to set up and use FACS for my sorting. How does set up time for the Pala Cell Sorter compare to traditional FACS?

設(shè)置和使用FACS進(jìn)行分類(lèi)非常耗時(shí)。Pala 細(xì)胞分選儀的設(shè)置時(shí)間與傳統(tǒng) FACS 相比如何？

Preparing and maintaining the instrument before and after the actual experiment—also known as overhead time—is the Achilles heel of many standard analytical instruments. The Pala Single Cell Dispenser drastically reduces overhead time, letting you go from initializing the instrument to achieving single cell isolation in as little as 7 minutes. This is in contrast to conventional FACS systems, which take over an hour to operate and provide results.
在實(shí)際實(shí)驗(yàn)之前和之后準(zhǔn)備和維護(hù)儀器（也稱(chēng)為開(kāi)銷(xiāo)時(shí)間）是許多標(biāo)準(zhǔn)分析儀器的致命弱點(diǎn)。Pala 單細(xì)胞分液器大大縮短了開(kāi)銷(xiāo)時(shí)間，讓您在短短 7 分鐘內(nèi)完成從初始化儀器到實(shí)現(xiàn)單細(xì)胞分離的整個(gè)過(guò)程。這與傳統(tǒng)的FACS系統(tǒng)形成鮮明對(duì)比，后者需要一個(gè)多小時(shí)才能運(yùn)行并提供結(jié)果。

FACS and limited dilution often yield few viable cells. Can the Pala Single and Bulk Cell Sorter help me achieve healthy cells after sorting?

FACS和有限的稀釋通常產(chǎn)生很少的活細(xì)胞。Pala 單體和散裝細(xì)胞分選儀能否幫助我在分選后獲得健康的細(xì)胞？

You can rest assured that the cells you get from the Pala system will be intact, healthy, and viable. The Pala system is gentle and uses very low pressure (<2 psi) for cell sorting, in contrast to high pressures (30-70 psi) typically used by conventional FACS sorters. The figure on the left shows a comparison of cellular stress response to pressures from FACS and Pala. Cells sorted with a FACS instrument showed induction of cellular stress response genes such as Ubiquitin C (Ubc), likely owing to high-pressure sorting conditions, while cells sorted on the Pala system showed much lower expression of these genes.
您可以放心，您從 Pala 系統(tǒng)獲得的細(xì)胞將是完整、健康和可行的。 Pala 系統(tǒng)非常溫和，使用非常低的壓力（<2 psi）進(jìn)行細(xì)胞分選，而傳統(tǒng) FACS 分選機(jī)通常使用的高壓（30-70 psi）。左圖顯示了細(xì)胞應(yīng)激反應(yīng)對(duì) FACS 和 Pala 壓力的比較。用FACS儀器分選的細(xì)胞顯示出細(xì)胞應(yīng)激反應(yīng)基因的誘導(dǎo)，如泛素C（Ubc），這可能是由于高壓分選條件，而在Pala系統(tǒng)上分選的細(xì)胞顯示出這些基因的表達(dá)要低得多。

Having to sort my prepared cells in a different lab complicates my experimental procedure and adds uncertainty to the outcome.

必須在不同的實(shí)驗(yàn)室中對(duì)我制備的細(xì)胞進(jìn)行分類(lèi)，使我的實(shí)驗(yàn)過(guò)程復(fù)雜化，并增加了結(jié)果的不確定性。

Pala is a compact, benchtop system which can easily fit into your tissue culture hood for sterile sorting. You can prepare your cells and easily isolate them in the same workstation!
Pala 是一種緊湊的臺(tái)式系統(tǒng)，可以輕松安裝到您的組織培養(yǎng)罩中進(jìn)行無(wú)菌分選。您可以在同一工作站中制備細(xì)胞并輕松分離它們！

The cell sorting step that happens on a FACS sorter exposes my cells to contamination risks. How does the Pala cell sorter mitigate contamination risk in my lab?

在FACS分選儀上進(jìn)行的細(xì)胞分選步驟使我的細(xì)胞面臨污染風(fēng)險(xiǎn)。Pala 細(xì)胞分選儀如何降低實(shí)驗(yàn)室的污染風(fēng)險(xiǎn)？

Pala and other single cell sorters from Namocell utilize disposable microfluidic cell cartridges. Specifically designed for sterile isolation, each cartridge is a closed system, preventing cross contamination and aerosol formation.
Pala 和 Namocell 的其他單細(xì)胞分選儀使用一次性微流控細(xì)胞盒。專(zhuān)為無(wú)菌隔離而設(shè)計(jì)，每個(gè)濾芯都是一個(gè)封閉系統(tǒng)，可防止交叉污染和氣溶膠形成。

I am concerned about learning how to use a complex instrument.

我擔(dān)心學(xué)習(xí)如何使用復(fù)雜的儀器。

The Pala system is automated and easy-to-use. It streamlines many of the steps required for traditional single cell sorters, therefore it doesn’t require any extensive training or specialized expertise. Anyone can run it!
Pala 系統(tǒng)是自動(dòng)化且易于使用的。它簡(jiǎn)化了傳統(tǒng)單細(xì)胞分選儀所需的許多步驟，因此不需要任何廣泛的培訓(xùn)或?qū)I(yè)知識(shí)。任何人都可以運(yùn)行它！

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